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If NNMT inhibition both restores adipocyte energy expenditure (promoting fat reduction) and supports myogenic differentiation (preserving or enhancing muscle), the net body composition effect could be recomposition simultaneous fat loss and lean mass preservation

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Previous work has shown that the abundance of Lactobacillus reuteri (normalized to a control group) decreases during the onset of obesity and remains suppressed even after dieting in mice 99 , which parallels our observations that vehicle-treated diet-switched mice had lower levels of Lactobacillus than the 5A-1MQ-treated mice, the latter of which also demonstrated greater weight loss

Moreover, insulin-like growth factor binding protein related proteins (IGFBPrPs) have been recently characterised, which aid the metabolic effects of the hormone but their role remains unclear [96, 97]

Results may vary depending on individual metabolism, baseline nutrient levels, and overall health

Eight weeks is a long enough window to observe effects, and four weeks off is a real break that gives the methylation cycle time to normalize before the next block