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Cells were suspended in 800 L of buffer A (10 mmol/L HEPES-KOH with 1.5 mmol/L MgCl 2 , 10 mmol/L KCl, 0.2 mmol/L phenylmethylsulfonyl fluoride, and 5 mol/L DTT) containing protease inhibitors (5 g/mL each of pepstatin, leupeptin, and aprotinin), and homogenized in a 10-mL Wheaton homogenizer

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Indeed, PTU ameliorated liver damage to a lesser extent than rutin and aescin, consistent with the recent study by Zhao et al

This phenomenon of lower and shorter-term sales volume makes antibiotics a less profitable investment for pharmaceutical companies