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Endotoxin testing is relevant for cell-culture use because bacterial lipopolysaccharide contamination can confound experiments and harm sensitive cell lines

They allow the incorporation of antigens, adjuvants, and surfactants, improving the magnitude and quality of immune responses [102]

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While it may be possible to generate microglia-containing COs, these spontaneous formation, self-directed protocols are not yet robust and well established in terms of cell ratio and heterogeneity between patient iPSC lines