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Therefore, quercetin could be one of the promising drugs in the treatment of renal disorders

doi: 10.1038/s41467-019-08680-6 182 WangC

The enhanced susceptibility of metZ to Pyrazinamide (PZA), a prodrug that gets activated upon exposure to acidic pH, further confirms the presence of a relatively acidic cytosol and accentuates the role of metZ in maintaining the pH homeostasis in M

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Alexa Fluor 488 Annexin V/Dead cell apoptosis assay Dead cell apoptosis assay is quick and suitable method for identification of apoptotic cells as Annexin V, being phospholipid binding protein has more affinity towards phosphatidyl serine, which moves from inner plasma membrane leaflet to outer leaflet in apoptotic cells and thereby these apoptotic cells can be quantitatively estimated [40] For this assay, Alexa Fluor 488 Annexin V/Dead cell apoptosis kit (Thermo Fischer Scientific, USA) was used

FUN14 domain-containing protein 1 (FUNDC1), a protein localized on the outer mitochondrial membrane, is associated with mitophagy and mediates the formation of mitochondria-associated endoplasmic reticulum membranes, which can lead to increased cytoplasmic levels of Ca 2+