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28.2% in controls) Improved collagen organization and increased deposition Enhanced glutathione levels and antioxidant enzyme activity Increased angiogenesis through VEGF and FGF-2 stimulation Reduced pro-inflammatory markers (TNF-, IL-6) with decreased NF-B signaling Fibroblast migration stimulation and extracellular matrix remodeling GHK-Cu research consistently demonstrates that the peptide is biologically active at very low concentrations in the nanomolar range (110 nM)

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Viswanathan V, Sivakumar S, Sai Prathiba A, Devarajan A, George L, Kumpatla S

The filtrated protein was applied to a Superdex, 26/60, 75 pg (Cytiva, Freiburg, Germany) size-exclusion chromatography column pre-equilibrated in phosphate-buffered saline (PBS: 137 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 2 mM KH 2 PO 4 , pH 7.4)