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The cells were passaged weekly in a 1 to 3 ratio, using PBS (Medicago, Uppsala, Sweden) for washing and 0.25% Trypsin-EDTA (Sigma-Aldrich, Steinheim, Germany) for detachment

Mice were dosed daily and orally, with vehicle (0.5% methylcellulose/0.2% tween-80) or 25 mg/kg palbociclib

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Although significant confirmatory and control experiments are needed to validate the differential interactions reported with maze training in this study, future studies implementing this approach may provide further insight into the acute effects of spatial learning and memory on the hippocampal proteome and on specific proteinprotein interactions that may affect cognitive function, particularly with increasing age