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In addition, a recent study indicated that the active hepatoprotective ingredient tetramethylpyrazine inhibits the activation of RIPK1/RIPK3 necrosome and the phosphorylation of MLKL caused by alcohol intake, and exerts an anti-ALD effect by alleviating necroptosis (Zhou et al., 2022b)

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By contrast, other evidence indicates that Cu+ may promote the formation of soluble toxic oligomers or redox cycling with Cu2+, contributing to oxidative stress and aggregation (192,193)