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Using in silico design, structureactivity relationships (SARs), chemical optimization, cell-based assays and in vivo experiments, we identified and characterized the first-in-kind small-molecule inhibitors of STX7Munc13-4-dependent endosomal maturation and signaling for use in immune cells that contribute to systemic inflammation

For instance, the observed mitigation of microgliosis and pro-inflammatory cytokine release could be directly investigated by co-culturing our hCiPSC-NSCs or their conditioned medium with activated microglial cells (e.g., BV-2 cell line or primary microglia stimulated with LPS/ATP)

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