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Elution of specifically bound phage particles from glutathione Sepharose was carried out with glutathione buffer (20 mM glutathione, 100 mM Tris, 200 mM NaCl, 0.1% Tween 20, pH 8.0) or for Ni-NTA agarose was carried out competitively with buffer with 500 mM imidazole (500 mM imidazole, 50 mM Na 2 HPO 4 , 300 mM NaCl, 0.1% Tween 20, pH 7.5)

Disruptions caused by this imbalance can lead to abnormal cellular processes that impair sperm quality and reduce their fertilizing ability [4, 22]

After 30 min, the lysate was clarified by centrifugation (1500 g ), filtered (0.22 m filter), aliquoted and the E1E2-containing lysates were stored at 80 C

In summary, how to specifically inhibit the amino acid metabolism of tumor cells and immunosuppressive cells in the microenvironment, and enhance the amino acid metabolism of anti-tumor immune cells such as CD8 + T cells, is an urgent problem to be solved

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(2012) Guidelines for the use and interpretation of assays for monitoring autophagy