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Smad4 and FAST-1 in the assembly of activin-responsive factor

Step 3: tert-Butyl 4-(6-((7-cyclopentyI-6-(dimethylcarbainoyI)-7H-pyrrolof2,3- d]pyrimidin-2-y l)amino)pyridin-3-yl-2,4,5-d^iperazine- 1 -carboxylate A mixture of tert-butyl 4-(6-bromopyridin-3-yl-2,4,5-dj)piperazine-l -carboxylate (150 mg, 0.43 mmol) and 2-amino-7-cyclopentyl-N,N-dimethyl-7H-pyrrolo[2,3-d|pyrimidine- 6- carboxamide (120 mg, 0.44 mmol), Pd(dba) 2 (12.6 mg, 0.022 mmol), BINAP (20 mg, 0.032 mmol) and CS2CO3 (420 mg, 1.29 mmol) in dioxane (3 mL) is degassed and heated to 1 10 C over a period of 24 hours

Prepared vials should typically be stored refrigerated between 2-8C for best stability

Waberzinek G, Markov J, Mastik J

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