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Neurobiol Dis (2012) 46:62534

6 On top of this, these effects were found to be short-lived

Purchase from reputable sources, ensure proper refrigeration, and cook thoroughly to an internal temperature of at least 70C to eliminate potential pathogens

Different substrates can be used, such as p-nitrophenyl phosphate (p-NPP) or 5-bromo-4-chloro-3-indolyl phosphate (BCIP) (78,79)

Equal amounts of cell protein lysates were separated by SDS PAGE and transferred to PVDF membranes (Millipore, Darmstadt, Germany)

Blank was the reaction mixture, and positive control was the reaction mixture illuminated in light for the same time, without extract