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The next day, cells were transfected with siRNA by combining 10l of 20M siRNA and 5L Dharmafect (Dharmacon, T-2001-03) in 800L OptiMEM (Gibco, 31985-062) and adding to a 6-well plate in RPMI media to a total volume of 4mL, for a final siRNA concentration of 50nM

Second, several studies reported an association between elevated circulating homocysteine and mortality in patients with coronary heart disease independent of conventional risk factors (Alfthan et al, 1997

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