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R.et al (2015)

The second reaction mixture comprised 2.0 l dNTPs (2.5 mM), 0.25 l Taq polymerase (0.05 U), 2.5 l reaction buffer IB (1x), 1.0 l of a solution containing the mixed primers (10 mM) and 16.75 l sterile ultra-pure water

The second one is the colonization of the gastrointestinal tract, which takes place from the first days of life till the 3 years of life, then intestinal biodiversity is formed and stabilized in later life (87)