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Use of vitamin B12 in the treatment and prevention of nitroprusside-induced cyanide toxicity

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10.1073/pnas.0403412101 Proc

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Immunoprecipitation Hepatocytes were lysed in an immunoprecipitation (IP) buffer (150 mM NaCl), 50 mM Tris-HCl, 1% Nonidet P-40, pH 7.8, and a mammalian cell-specific protease inhibitor cocktail

1 Introduction Oxidative stress (OS) happens when there is an uneven balance between harmful oxidative processes and protective antioxidants in the body