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2.3 Metabolites analysis 2.3.1 Determination of urinary creatinine Before testing, the samples were naturally thawed at room temperature, then the urine sample was centrifuged at 12000 rpm for 5 min, and the supernatant was ready for use

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Serum limitation-induced senescent fibroblasts model and effect of FOXO4-DRI Passage 3 fibroblasts derived from keloids and normal skins were cultured in a Dulbeccos Modified Eagles medium (DMEM, Hyclone, Logan, Utah, USA) containing 10% fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) to 90% confluence, and then cultured in a DMEM containing 2% FBS for 3 days to construct a serum limitation-induced senescent fibroblast model

Peptides need time to penetrate the scalp

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