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Fortunately, most of the calculated doses used in this study align with those suggested in many previous studies

For example, cancer cells often upregulate proteins that increase intracellular iron levels, such as TFR1, IREB2, STEAP3, and hepcidin [18, 19]

doi: 10.1172/JCI70454 157 DasCKMandalMKgelD

The spleens were manually dissociated, and the cells were cleaned by passage through a 70 m strainer followed by a 2-minute incubation in ACK medium (37 mM NH4Cl, 10 mM KHCO3, 0.1 M EDTA) at room temperature