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For saturation binding experiments, we incubated cells with increasing concentrations of ghrelin-A2 in the presence or absence of 10 M unlabeled ghrelin for 1 hour at R.T

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(C) In vitro palmitoylated GSTP1 was treated with NaOH (0.1 M) or MOPS at pH 7 for 1 h and then analyzed

These parameters provide insights into the conformational stability and interactions of each ligand within the proteinligand complex

14 CorpechotC.BarbuV.WendumD.ChignardN.HoussetC.PouponR.et al (2002)

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