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After this initial culture and application of physical (e.g., stretch) and chemical (e.g., O 2 ) conditions, the cells were exposed to FW2 NPs at different concentrations (5, 7.5, and 10 g/cm 2 ) for 46 h

The CRISPR-Cas9-initiated fixed-point strategy was employed to successfully incorporate spider silk protein genes into the Bombyx mori genome, resulting in fibers that are as strong as native spider silk, with a high tensile strength of 1.2 GPa [84]

135 min gradient) and acquired in DDA mode (MS1: 3502000 m/z, followed by MS/MS)

[DOI] [PubMed] [Google Scholar] 184.Lee KS

and the content of proteins was quantified with BCA assay (Thermo Fisher Scientific, Waltham, MA, USA)

Taska K, Gietka-Czernel M, Glinicki P, Kozakowski J