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In many cases, the issue is physical rather than a direct sign of poor peptide quality

To generate adh-5 null mutant, we utilized a universal STOP-IN cassette that contained an exogenous Cas9 target site, multiple stop codons in all three reading frames, and the recognition site of the NheI restriction enzyme 23

Yao et al., 2013, 2016)] the expression became nearly undetectable (Figure 1) and prevents the discharge of SELENOP in the urine (Olson et al., 2008

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The mice in this study were placed on a maximally tolerable restriction of both calories and protein which was accompanied by an extreme loss of up to 50% body weight