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J Biol Chem 269(20):1483514840

At mentioned time points, MTT solution was added, incubated in darkness for 4 h at 37 C, and then, replaced by dimethyl sulfoxide to dissolve the formazan crystals and finally absorbance was read at 570 nm by a plate reader

& Szumlinski, K

FDA and by other regulatory agencies for the treatment of amyotrophic lateral sclerosis, in which it has a modest but clear beneficial effect (Miller, Mitchell, Lyon, & Moore, 2007)