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654 Of note, astrocyte-specific deletion of mutant huntingtin in mice globally expressing this gene eased disease symptoms and delayed its progression, indicating a role for astrocytes in the pathophysiology of the disease

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[DOI] [PubMed] [Google Scholar] 183.Zhu L., Baker S.S., Gill C., Liu W., Alkhouri R., Baker R.D., Gill S.R

Gene knockout by CRISPR/Cas9-sgRNAs The sgRNA expression plasmids targeting human SLC13A3 (sgRNA: GATGGTGACAATAGCCACGC) or mouse Slc13a3 ( sgRNA: GGAAGGAGGATGAATATCGT) were constructed by ligating the corresponding annealed guide oligonucleotides to pLenti-U6-sgRNA-EFFS-spCas9-P2A-Puro plasmid co-expressing the Cas9

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Even in genetically identical cancer cell populations, this process leads to the spontaneous emergence of subpopulations with distinct epigenetic profiles, forming potential seeds for resistance [199,200,201]