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They were then washed with PBS on the rotating bed for 5 min and permeabilized using 0.1% Triton X-100 in PBS for 10 min, followed by another 5 min PBS wash on the rotating bed

Caliceti P, Schiavon O, Morpurgo M et al (1995) Physico-chemical and biological properties of monofunctional hydroxy terminating poly(N-vinylpyrrolidone) conjugated superoxide dismutase

Chromatin immunoprecipitation assays ChIP experiments were conducted using ChIP-IT High Sensitivity Kit (ab185913, Abcam) with adaptations