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Redox Biol 5:367380

Although a limitation is that halogenated compounds containing chloride ions (e.g., Tris-HCl, NaCl) cannot be used in the lysis or binding buffers, the system offers the advantage of one-step purification (~1 h) with high specificity, as has been reported in several applications involving recombinant protein expression and purification using this system [43,44,165,166]

Pat the area dry with a clean towelnever rub vigorously

It's really great when we can reverse those markers, especially for the time capsule for the baby, but also for some of the risk factors for pregnancy loss