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Trypsin (TPCK-treated, from bovine pancreas), pepsin (from porcine gastric mucosa), dithiothreitol (DTT), acetaminophen (APAP), HPLC-grade acetonitrile (ACN) and methanol (MeOH), iodoacetamide (IAM), glucose-6-phosphate dehydrogenase (type XV, from baker's yeast), and other chemicals were purchased from Sigma-Aldrich (St

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The energy released by the transport of electrons is utilized to expel protons (H +) from the mitochondrial matrix across the mitochondrial inner membrane (at multisubunit protein complexes include complex I, complex III, and complex IV) into the intermembrane space [12]

This means it is made of three specific amino acids: cysteine, glycine, and glutamic acid

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