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Description

Confocal microscopic examination of acetylated -tubulin (Ac-Tub)labeled cilium was used to determine the length of cilia in the GFP + -transfected cells and the percentage of GFP + -ciliated cells

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Additionally, the visual examination of the pellets color after centrifugation was checked to determine whether the dye had been adsorbed to the cells rather than being degraded