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Y.et al (2025)

However, ex vivo genome-wide expression profiling of microglia from different disease model mice failed to support the M1/M2 polarization theory (Chiu et al., 2016

Tissues were immersion-fixed in 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4) for 24 h at 4 C, dehydrated through graded ethanol, cleared in xylene, and embedded in paraffin