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Therefore, both WGS metagenomic analysis and traditional marker-based metagenomics using next-generation sequencing provide culture-independent approaches to comprehensively characterize the human gut microbiome and environmental microbial communities

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The gastrocnemius muscles were then excised, washed in cold phosphate-buffered saline, snap-frozen in liquid nitrogen-cooled isopentane, and stored at 80 C until analysis [2]