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Int J Mol Sci 24(9):8323 Gygi SP, Rist B, Gerber SA, Turecek F, Gelb MH, Aebersold R (1999) Quantitative analysis of complex protein mixtures using isotope-coded affinity tags

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Death cells were calculated as: the necrosis and late apoptosis cells (Q2) + early apoptosis cells (Q3)

50 mM potassium phosphate buffer (pH 7.0), 0.5 mM ascorbic acid, 0.1 mM EDTA, 0.1 mM H 2 O 2 , 0.1 mL enzyme and water to make a final volume of 3.0 mL in which 0.1 mL of H 2 O 2 was added to initiate the reaction