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After completion, as monitored by UPLC and ESI-LCMS, the reaction mixture was diluted with buffer A, filtered and purified directly by preparative RP-HPLC, eluting with a linear gradient (see the Peptide cleavage and purification section)

Benefits of adequate vitamin D are also extensive and include bone/teeth, hormone, mood, skin, immune, lung, metabolic, cardiovascular, liver and kidney health

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In the Roman period it decreases drastically from 14% to less than 1%, then remaining infrequent despite a weak increase during Late Antiquity and the Early Medieval period (ad 7501000) (Fig