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Lipoxygenase Lipoxygenases (LOs) are a family of enzymes that catalyze the addition of oxygen to polyunsaturated fatty acids (PUFAs), specifically those containing a 1,4-pentadiene structure [245]

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Cells were then incubated in ChIP lysis buffer (50 mM Tris-HCl pH 8.0, 5 mM EDTA, 150 mM NaCl, 1% NP-40, 0.1% SDS, with protease inhibitor) for 30 min on ice