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Together, both our in vitro and cell-based assays show that the -loop region of GDAP1 plays a critical role in 4HNE recognition and GDAP1s cytoprotective function in the cell

This also highlights the potential influence of tumor metabolic status (e.g., glycolysis activity) on cuproptosis sensitivity low LDH levels correlate with impaired glycolysis and enhanced mitochondrial metabolism, thereby fostering the metabolic dependencies of cuproptosis

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(K) Western blot analysis of p-p38, p38, CyclinA2, IL6, and Flag on day W11 in NCI-H446 cells overexpressing Flag-CRIP1 WT/R16K/R16F with Ana from W8 to W11