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Prior to the coating with FN, multi-well plates or flasks were washed three times with sterile water to remove excess PLO

Documented protocols keep it at 1-2mL

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Disclosure of interest: no E-Posters - RISK FACTORS - PRIMARY AND SECONDAR PREVENTION 05.00 - RISK FACTORS AND PREVENTION (PRIMARY AND SECONDARY) - 05.03 - EPIDEMIOLOGY & RISK FACTORS P2551 - ESOC25-1989 RISK FACTORS AND PREDICTION MODEL OF RECURRENT STROKE FOR PATIENTS WITH SYMPTOMATIC INTRACRANIAL ARTERIAL STENOSIS AND TREATED WITH MEDICAL THERAPY Yixin Sun 1,2 , Ruofan Li 3 , Eyad Almallouhi 4 , Tao Wang 5 , Jie Wang 5 , Jichang Luo 5 , Colin P Derdeyn 6 , Liqun Jiao 5 1 Department of Neurosurgery, Xuanwu Hospital, Beijing, China, 2 Eight-year-program, Grade 2018, Peking Unversity First Hospital, Beijing, China, 3 Health Science Center, Peking University, Beijing, China, 4 Departments of Neurology and Neurosurgery, Medical University of South Carolina, Charleston, United States, 5 Capital Medical University Xuanwu Hospital, Beijing, China, 6 Departments of Radiology and Neurology, University of Iowa Hospitals and Clinics, Iowa City, United States Background and Aims: Intracranial atherosclerosis is a common cause of stroke, with a high risk of recurrent stroke

Mller TD, Clemmensen C, Finan B, et al

These include familial Alzheimer's disease, SCA17 (dominant inheritance)