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For the immunostaining of cultured PSCs, cells were fixed in 4% PFA for 15 min followed by a 15-min incubation in 0.5% Triton-X100

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In contrast, bilirubin, which is a strong regulator of PPAR (13) and PPAR (178), can also trigger the expression of FABP1 through the regulation of PPAR

Before imaging, a drop of the tested dispersion was applied to a carbon-coated copper grid, colored with phosphotungstic acid, and allowed to dry in the air