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[DOI] [PMC free article] [PubMed] [Google Scholar] 79.Kaelle GCB, Souza CMM, Bastos TS, Vasconcellos RS, de Oliveira SG, Felix AP

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Total protein was extracted using the ice-cold extraction buffer containing 100 mM potassium phosphate buffer, pH 7.0, 50% glycerol, 16 mM MgSO 4 and 1 mM PMSF 57