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All mice were enrolled in the study in numerical order and randomly allocated to experimental groups by lottery, with the group allocation performed by a third-party investigator to ensure that the experimenters remained blinded to group assignments

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It is well-established that granulosa cells apoptosis is the primary driver of atresia in growing follicles, while oocyes apoptosis contributes to primordial follicular atresia

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These secondary mechanisms operate in addition to not instead of the primary VEGFR2 and NO pathways