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Afterwards, cells were washed with PBS, and incubated at room temperature for 30 min with an anti-rabbit IgG Alexa Fluor 546 antibody (1:100, #A-11035, Thermo Fisher Scientific, Waltham, MA, USA)

Data availability The datasets generated and analyzed during the current study are available and attached to this submission

2013;2(6):e000364

Association of tramadol with all-cause mortality among patients with osteoarthritis

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