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Therefore, the immunoreactive melatonin also was measured in the rat gastro-intestinal mucosa, the liver and the pancreas as well as in the portal, hepatic and arterial blood by specific RIA in tissue homogenate, blood plasma in tests without and with oral administration of tryptophan (100 mg/kg) (28)

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LP levels were determined by spectrophotometer (Shimadzu, Japan) at 532 nm

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