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Front Neurosci 5:80 Blasi E, Barluzzi R, Bocchini V, Mazzolla R, Bistoni F (1990) Immortalization of murine microglial cells by a v-raf/v-myc carrying retrovirus

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Altered firing output of VIP interneurons and early dysfunctions in CA1 hippocampal circuits in the 3xTg mouse model of Alzheimers disease

The samples were incubated at 37 C for both 15 and 75 minutes before being washed twice in culture medium and fixed as described previously