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5 and 6, samples in SDS solubilizer (0.0625 M TrisHCl (pH 6.8), 10% glycerol, 0.01% bromophenol blue, 2% (wt/vol) SDS, +/- 2% 2-mercaptoethanol) were heated at 95 C for 5 min, electrophoresed through 8% or 10% (wt/vol) polyacrylamide-SDS gels, transferred to nitrocellulose membranes (Bio-Rad, USA), and incubated with SARS-CoV-2 (2019-nCoV) S antibody rabbit PAb antigen affinity purified, mouse monoclonal anti-SARS-S2 conjugated to HRP, goat anti-human IgG-HRP, ACE2 recombinant rabbit monoclonal antibody, or purified LgBiT-substrate cocktail

For the determination of non-enzymatic antioxidants, fresh leaves (0.5 g) were extracted using 3 mL of ice-cold 5% meta-phosphoric acid containing 1 mM EDTA, and then the extracted solution was centrifuged at 11,500g for 15 min

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833 HMGB1 is a highly conserved nuclear protein

Picha, Optimization of lycopene extraction from tomato pomace and effect of extract on oxidative stability of peanut oil

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