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Description

7) or (ii) from intramitochondrially produced acetate from pyruvate, mediated by neomorphic activity of pyruvate dehydrogenase complex or other keto-acid dehydrogenases and in the presence of ROS 169,170,252,253,284 (Fig

Fibroblasts (4,000 cells/well) were seeded in black-walled 96-well plates and, after 24 h, loaded with Fluo-8E AM for 45 min at 37 C

prsente dans chaque cellule, sa fonction principale est d'aider la conversion des aliments en nergie

Given that almost identical IC50 values for MTX were obtained by MTT and calcein AM at 48 and 96 hours, either one or the other method was used to access cell viability, depending on experimental and laboratory logistics