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Researchers do this to equalise pressure and make drawing easier, but it introduces airborne contaminants and establishes a positive pressure differential that forces solution back through the needle tract on every subsequent draw

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Treatment with the transition-metal chelating agents deferoxamine and trientine, which can prevent auto-oxidation, corrected nerve conduction and blood flow changes that occurred 2 months after the induction of diabetes in this model [28]

This exchange supports glutathione synthesis, protects cells from oxidative stress, and maintains cellular redox balance (Hong et al., 2021)