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When the 1:5 ratio was administered, the acidosis was less marked, but still three of seven animals succumbed within 24 h

Pure distilled water is not appropriate for injection-based research applications at all

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For best results, you might allow the vial to sit at room temperature for a few minutes before reconstitution to avoid a thermal shock to the lyophilized powder, but the water itself will work just as effectively.

J Bone Jt Surg Am 92:19271937 Fortier LA, Strauss EJ, Shepard DO, Becktell L, Kennedy JG (2019) Biological effects of bone marrow concentrate in knee pathologies