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Following several iterations to induce self-assembly of the three populations into a single structure, we found that mixing one h-HepOrg structure with a defined number of portal fibroblasts and cholangiocytes (from h-CholOrgs), from the same donor when possible, in 96-well low-adhesion U-bottom plates, readily generated structures in which the three cell types were together, with cholangiocytes and portal fibroblasts embedded inside the h-HepOrg structure