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This broad term ignores the fact that the biology of individual types of ROS is highly diverse, and their chemical reactivity and second-order rate vary significantly ( to form the highly oxidative peroxynitrite (ONOO - ) ( 2 O 2 , , and OH- play important roles in the redox regulation of biological activities ( is decomposed to H 2 O 2 by the action of superoxide dismutase (SOD), which can be further reduced to H 2 O or OH-

Concentrations of GSH and GSSG were determined as described previously (Grant et al ., 1998)

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Biological analyses were performed on plasma samples, quantifying total and reduced glutathione, oxidized glutathione and redox ratios using HPLC with colorimetric detection, while behavioral outcomes were assessed weekly through standardized caregiver questionnaires and clinician-rated scales

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