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All these approaches are reported to have a less-negative impact on EF, with many of the studies reporting a complete recovery at one-year follow-up [318]

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Proteomic analysis In-solution trypsin digestion: 200 L of solution were mixed with 100 L of 25 mM ammonium bicarbonate buffer pH 8.5, reduced by adding 30 L of 10 mM DTT and incubated at 56 C for 20 min