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Oral intake of GABA supplements may improve sleep quality by promoting relaxation and reducing anxiety, potentially beneficial for individuals with insomnia (Yoto et al., 2012

pmid:21840335 3

Xiong Z, Liu X, Wang Q

Fig.2 GST tag resin GST resin technical parameters Buffer recommendation Equilibration buffer: 10-20mM PB/50-100mM Tris-HCl + 0.14-0.4M NaClpH6.2-8.0 Elution buffer50mM Tris-HCl+10-60mM reduced glutathione, pH8.0-9.0 Tips: To improve purity of target molecules, add reducing agent in binding buffer and elution buffer, i.e