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siRNA and plasmid transfection For siRNA transfection, cells were transiently transfected with target-specific siRNA oligonucleotides (GenePharma) using the JetPRIME transfection reagent (Polyplus, Bas-Rhin, France) in accordance with the manufacturers protocol, and downstream assays were performed 48 h post-transfection

When bulls were fed a diet rich in omega-3 PUFAs, the altered FAs profile in the sperm plasma membrane made the membrane more resistant to damage caused by ice crystal formation during freezing (66)

Studies were excluded if they did not directly pertain to these areas or if they were not in the English language

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Some appear as red lines across the skin, while others may look white or silvery