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Because the placenta possesses the ability to sense and adapt to variations in the gestational environment, malfunction of the placenta has been apparently associated with complications in pregnancy [9]

Afterwards (D3-D4), cells were split using the Trypsin/EDTA solution (BioWest) and seeded for experiments

In all our previous murine studies we have used short cycles (1014 days) of very low subcutaneal doses (20 g/kg/day) after carefully assessing its circulating concentrations and using the minimum amount that was sufficient to adequately restore physiological values of the molecule