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For histo-morphometric evaluation, the parameters namely seminiferous tubule diameter (STD), thickness of the germinal epithelium of the ST (from the basement membrane to lumen), spermatogonia (Sg), preleptotene, spermatocytes (PLSc), pachytene spermatocytes (PSc), and spermatid cells (Sd) observed in 10 tubules per testicular section and 10 section per groups were measured at 40 magnifications by using calibrated OLYSIA Soft Imaging System GmbH, version 3.2 (Japan).36 Ethical considerations All experimental procedures followed the recommendations provided in the Guide for the Care and Use of Laboratory Animals prepared by the National Academy of Sciences and Published by the National Institute of Health.37 Data presentation and statistical analysis Data were expressed as MeanSEM

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In total, 4070% reduction in total IgG and AChR antibody levels were noted within the first week of the initial dose, recovering by 12 weeks and the levels corresponded inversely with clinical improvement

67 68 Long term epidemiological studies also indicate that treatment with pharmacological B 12 doses and the corresponding increase in serum B 12 do not increase mortality

Mounting evidence has emerged in gut microbiota as a novel therapeutic target for the treatment of CVDs

[DOI] [PubMed] [Google Scholar] Wang, L