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This imbalance sustains a self-amplifying loop of oxidative stress, neuroinflammation, and mitochondrial dysfunction, reinforcing chronic pain pathophysiology

FIGURE 8 3.8 Immunohistochemical findings The immunohistochemical investigation demonstrated a significant increase in expression of proapoptotic makers, Bax, and caspase-3, in MTX treated group, which was associated with decreased antiapoptotic marker, Bcl-2, expression when compared to the CTRL group (Figure 9)

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